Construction of plasmids expressing recombinant B cell epitopes of PD1
نویسندگان
چکیده
منابع مشابه
Construction of recombinant Pichia pastoris expressing single-chain antibody fragment against extracellular domain of EpCAM
Introduction: Epithelial cell adhesion molecule (EpCAM) is highly expressed on epithelial tumors. So, EpCAM is a valuable antigen for targeted therapy. Using monoclonal antibodies (mabs) is an attractive approach for targeted cancer therapy. Importantly, limitations of intact mabs including large size led to the development of antibody fragments such as single chain fragment variable (scfv). Pi...
متن کاملConstruction of recombinant yeast expressing EpEX as a suitable candidate in cancer diagnosis and therapy
Introduction: Epithelial cell adhesion molecule (EpCAM) is a membrane glycoprotein that is overexpressed on the majority of tumor cells of epithelial origin and thereby can be used as a target of immunodetection and immunotherapy of cancer. So, it is important to produce this protein in its native form. Interestingly, during the last two decades, the yeast Pichia pastoris (P. pastoris) has beco...
متن کاملRemoval of B-cell epitopes for decreasing immunogenicity in recombinant immunotoxin against B-cell malignancies.
Recombinant immunotoxin HA22, composed of an anti- CD22 Fv fragment fused to PE38, a truncated portion of Pseudomonas Exotoxin A (PE), has been developed for targeted treatment of various B-cell malignancies. As a foreign, internalized macromolecule, PE38 often induces lysosomal degradation and neutralizing antibodies to limit the efficacy of treating B-cell malignancies. The region of PE38 con...
متن کاملCONSTRUCTION OF RECOMBINANT PLASMIDS FOR PERIPLASMIC EXPRESSION OF HUMAN GROWTH HORMONE IN ESCHERICHIA COLI UNDER T7 AND LAC PROMOTERS
In order to study the periplasmic expression of human growth hormone (hGH) in Escherichia coli, the related cDNA was inserted in two expression plasmids carrying pelB signal peptide, one with lac bacterial promoter and the other with a bacteriophage T7-based promoter. The recombinant plasmids were moved to TG1 and BL21 strains of E. coli, respectively. To induce the expression systems, IPTG and...
متن کاملConstruction of Staphylococcus aureus Proline Transport Gene Recombinant Plasmids
The bacterial species Staphylococcus aureus is responsible for a significant amount of infections and disease in humans each year. The emergence of antibiotic resistance within the species is of great concern. A recent study has suggested that proline transport is important for S. aureus survival in mice. This previous study specifically examined the putP gene, which codes for a high affinity p...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Health Science Journal of Indonesia
سال: 2019
ISSN: 2338-3437,2087-7021
DOI: 10.22435/hsji.v10i1.1848